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Western Blot at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
IRX3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.

IRX3 Antibody

CATALOG NUMBER: 57-809

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Xenopus
Immunogen:
This IRX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the N-terminal region of human IRX3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IRX3
Additional Names:
Iroquois-class homeodomain protein IRX-3, Homeodomain protein IRXB1, Iroquois homeobox protein 3, IRX3, IRXB1
Protein Accession Number:
P78415
Protein Gi Number:
47117874
Ncbi Gene Id Number:
79191
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IRX3 is a member of the Iroquois homeobox gene family (seeIRX1; MIM 606197) and plays a role in an early step of neuraldevelopment (Bellefroid et al., 1998 [PubMed 9427753]). Members ofthis family appear to play multiple roles during pattern formationof vertebrate embryos (Lewis et al., 1999 [PubMed10370142]).
Background Reference 1:
Ragvin, A., et al. Proc. Natl. Acad. Sci. U.S.A. 107(2):775-780(2010)
Background Reference 2:
Trynka, G., et al. Gut 58(8):1078-1083(2009)
Background Reference 3:
Lewis, M.T., et al. Cell Tissue Res. 296(3):549-554(1999)
Background Reference 4:
Bellefroid, E.J., et al. EMBO J. 17(1):191-203(1998)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-809

List Size:
400 ul

List Price:

$495.00

Datasheet
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