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Western blot analysis of extracts of various cell lines, using ISG15 antibody (14-152) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human gastric cancer using ISG15 Antibody (14-152) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ISG15 Antibody (14-152) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using ISG15 Antibody (14-152) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ISG15 Antibody (14-152) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using ISG15 antibody (14-152) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ISG15 Antibody

CATALOG NUMBER: 14-152

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human ISG15 (NP_005092.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
PC-3
Positive Control 3:
SW620
Positive Control 4:
HCT116
Predicted Molecular Weight:
Observed: 14kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ISG15
Additional Names:
ISG15, HUCRP, IFI15, ISG15 ubiquitin-like modifier, G1P2, IP17, Ubiquitin-like protein ISG15, UCRP
Ncbi Gene Id Number:
9636
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-152

List Size:
100 uL

List Price:

$436.00

Datasheet
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