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Western Blot at 1:1000 dilution Lane 1: human heart lysate Lane 2: human skeletal muscle lysate Lysates/proteins at 20 ug per lane.
ISLR Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: human heart lysate Lane 2: human skeletal muscle lysate Lysates/proteins at 20 ug per lane.

ISLR Antibody

CATALOG NUMBER: 61-694

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ISLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-362 amino acids from the C-terminal region of human ISLR.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
46 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ISLR
Additional Names:
Immunoglobulin superfamily containing leucine-rich repeat protein, ISLR
Protein Accession Number:
O14498
Ncbi Gene Id Number:
3671
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background Reference 1:
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
Background Reference 2:
Nagasawa, A., et al. Genomics 61(1):37-43(1999)
Background Reference 3:
Nagasawa, A., et al. Genomics 44(3):273-279(1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-694

List Size:
400 ul

List Price:

$495.00

Datasheet
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