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Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

ISWI Antibody

CATALOG NUMBER: 55-929

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ISWI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 946-975 amino acids from the C-terminal region of human ISWI.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
122 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SMARCA5
Additional Names:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5, 364-, Sucrose nonfermenting protein 2 homolog, hSNF2H, SMARCA5, SNF2H, WCRF135
Protein Accession Number:
O60264
Ncbi Gene Id Number:
8467
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of theSWI/SNF family of proteins. Members of this family have helicaseand ATPase activities and are thought to regulate transcription ofcertain genes by altering the chromatin structure around thosegenes. The protein encoded by this gene is a component of thechromatin remodeling and spacing factor RSF, a facilitator of thetranscription of class II genes by RNA polymerase II. The encodedprotein is similar in sequence to the Drosophila ISWI chromatinremodeling protein.
Background Reference 1:
Goldman, J.A., et al. J. Biol. Chem. 285(7):4645-4651(2010)
Background Reference 2:
He, X., et al. Biochemistry 47(27):7025-7033(2008)
Background Reference 3:
Sheu, J.J., et al. Cancer Res. 68(11):4050-4057(2008)
Background Reference 4:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-929

List Size:
400 ul

List Price:

$495.00

Datasheet
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