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45-782 (0.01ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-782 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-782 (0.01ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ITF1 Antibody

CATALOG NUMBER: 45-782

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-KAPRARTSPDEDED
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in lysates of cell line Daudi (calculated MW of 67.6kDa according to NP_003191.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5ug/ml.
Specificity:
This antibody is expected to recognize reported isoform E12 (NP_003191.1) only.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TCF3
Additional Names:
TCF3, ITF1, transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47), E2A, MGC129647, MGC129648 , E2A immunoglobulin enhancer-binding factor E12/E47, immunoglobulin transcription factor 1, kappa-E2-binding factor, transcription factor 3, transcription factor E2-alpha
Protein Accession Number:
NP_003191.1
Protein Gi Number:
27777636
Ncbi Gene Id Number:
6929
Background Reference 1:
Greenbaum S, Lazorchak AS, Zhuang Y. Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes. J Biol Chem. 2004 Oct 22;279(43):45028-35. Epub 2004 Aug 13. Erratum in: J Biol

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-782

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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