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43-636 (0.5ug/ml) staining of HeLa (A) and HepG2 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-636 (0.5ug/ml) staining of HeLa (A) and HepG2 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ITGA1 / VLA1 Antibody

CATALOG NUMBER: 43-636

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DKHDFQDSVRIT
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 131kDa according to NP_852478.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
Predicted Molecular Weight:
Approx 150kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ITGA1 / VLA1
Additional Names:
ITGA1, integrin, alpha 1, CD49a, VLA1, CD49 antigen-like family member A, VLA-1, integrin alpha-1, laminin and collagen receptor, very late activation protein 1
Protein Accession Number:
NP_852478.1
Protein Gi Number:
2829468
Ncbi Gene Id Number:
3672
Background Reference 1:
Roberts AI, Brolin RE, Ebert EC.Integrin alpha1beta1 (VLA-1) mediates adhesion of activated intraepithelial lymphocytes to collagen.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-636

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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