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42-769 (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-769 (2ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
42-769 Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.
42-769 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-769 (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ITGAL Antibody

CATALOG NUMBER: 42-769

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-YQVRIQPSIHDHN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 200kDa band observed in Human Spleen and Tonsil lysates (calculated MW of 129kDa according to NP_002200.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.Immunohistochemistry:In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_002200.2; NP_001107852.1).
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 1315 - Human Tonsil Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ITGAL
Additional Names:
ITGAL, integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide), CD11A, LFA-1, LFA1A, LFA-1 alpha, antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide, integrin alpha L, integrin gene promoter, lymphocyte function-associated antigen 1
Protein Accession Number:
NP_002200.2,NP_001107852.1
Protein Gi Number:
167466215
Ncbi Gene Id Number:
3683

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-769

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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