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43-607 (0.3ug/ml) staining of K562 (A) and U937 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-607 (0.3ug/ml) staining of K562 (A) and U937 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ITGB2 / LFA-1 Antibody

CATALOG NUMBER: 43-607

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-ETQEDHNGGQKQLS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 90kDa band observed in lysates of cell lines K562 and U937 (calculated MW of 84.8kDa according to NP_00202.2). Recommended concentration: 0.3-1ug/ml.
Specificity:
Reported variants represent identical protein: NP_001120963.1, NP_000202.2.
Predicted Molecular Weight:
Approx 90kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ITGB2 / LFA-1
Additional Names:
ITGB2, integrin, beta 2 (complement component 3 receptor 3 and 4 subunit), CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7, cell surface adhesion glycoprotein LFA-1/CR3/P150,959 beta subunit precursor), cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, complement receptor C3 beta-subunit, complement receptor C3 subunit beta, integrin beta chain, beta 2, integrin beta-2, leukocyte cell adhesion molecule CD18, leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
Protein Accession Number:
NP_000202.2
Protein Gi Number:
124056465
Ncbi Gene Id Number:
3689
Background Reference 1:
Bolomini-Vittori M, Montresor A, Giagulli C, Staunton D, Rossi B, Martinello M, Constantin G, Laudanna C.Regulation of conformer-specific activation of the integrin LFA-1 by a chemokine-triggered Rho signaling module.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-607

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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