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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
ITGB5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

ITGB5 Antibody

CATALOG NUMBER: 57-409

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This ITGB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-244 amino acids from the N-terminal region of human ITGB5.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
88 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ITGB5
Additional Names:
Integrin beta-5, ITGB5
Protein Accession Number:
P18084
Protein Gi Number:
124970
Ncbi Gene Id Number:
3693
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Li, Z., et al. J. Biol. Chem. 285(31):23699-23710(2010)
Background Reference 3:
Leifheit-Nestler, M., et al. Arterioscler. Thromb. Vasc. Biol. 30(7):1398-1406(2010)
Background Reference 4:
Lane, D., et al. Oncogene 29(24):3519-3531(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-409

List Size:
400 ul

List Price:

$495.00

Datasheet
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