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Western blot analysis of extracts of various cell lines, using ITIH3 antibody (23-863) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using ITIH3 antibody (23-863) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

ITIH3 Antibody

CATALOG NUMBER: 23-863

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 35-180 of human ITIH3 (NP_002208.3).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
HepG2
Positive Control 2:
293T
Positive Control 3:
NCI-H460
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse lung
Positive Control 6:
Rat liver
Predicted Molecular Weight:
Observed: 105kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ITIH3
Additional Names:
H3P, ITI-HC3, SHAP, inter-alpha-trypsin inhibitor heavy chain H3, ITI heavy chain H3, inter-alpha (globulin) inhibitor H3, inter-alpha (globulin) inhibitor, H3 polypeptide, inter-alpha-inhibitor heavy chain 3, pre-alpha (globulin) inhibitor, H3 polypeptide, serum-derived hyaluronan-associated protein
Ncbi Gene Id Number:
3699
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex may stabilize the extracellular matrix through its ability to bind hyaluronic acid. Polymorphisms of this gene may be associated with increased risk for schizophrenia and major depressive disorder. This gene is present in an inter-alpha-trypsin inhibitor family gene cluster on chromosome 3.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-863

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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