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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
ITIH5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

ITIH5 Antibody

CATALOG NUMBER: 55-530

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ITIH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 578-606 amino acids from the C-terminal region of human ITIH5.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
105 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ITIH5
Additional Names:
Inter-alpha-trypsin inhibitor heavy chain H5, ITI heavy chain H5, ITI-HC5, Inter-alpha-inhibitor heavy chain 5, ITIH5, KIAA1953
Protein Accession Number:
Q86UX2
Protein Gi Number:
187609608
Ncbi Gene Id Number:
80760
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a heavy chain component of one of theinter-alpha-trypsin inhibitor (ITI) family members. ITI proteinsare involved in extracellular matrix stabilization and in theprevention of tumor metastasis. They are also structurally relatedplasma serine protease inhibitors and are composed of a light chainand varying numbers of heavy chains. This family member is thoughtto function as a tumor suppressor in breast and thyroid cancers.Alternative splicing results in multiple transcript variants.
Background Reference 1:
Pita, J.M., et al. Br. J. Cancer 101(10):1782-1791(2009)
Background Reference 2:
Veeck, J., et al. Pathologe 29 SUPPL 2, 338-346 (2008) :
Background Reference 3:
Veeck, J., et al. Oncogene 27(6):865-876(2008)
Background Reference 4:
Hamm, A., et al. BMC Cancer 8, 25 (2008) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-530

List Size:
400 ul

List Price:

$495.00

Datasheet
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