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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human (ITK) with DYKDDDDK tag probed with 45-784 (1ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/10000) in lane C. Mock-transfected HEK293 probed with EB10338 (1mg/ml) in Lane B. Primary
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human (ITK) with DYKDDDDK tag probed with 45-784 (1ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/10000) in lane C. Mock-transfected HEK293 probed with EB10338 (1mg/ml) in Lane B. Primary

ITK Antibody

CATALOG NUMBER: 45-784

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-RLLRQLAEIAESGL
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:In transfected HEK293 transiently expressing full-length Human ITK (myc and DYKDDDDK tagged), a band of approx. 75kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti- DYKDDDDK tag antibody. Recommended concentration, 1-2ug/ml.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ITK
Additional Names:
ITK, EMT, LYK, PSCTK2, IL2-inducible T-cell kinase, tyrosine-protein kinase LYK, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, MGC126257, MGC126258
Protein Accession Number:
NP_005537.3
Protein Gi Number:
15718680
Ncbi Gene Id Number:
3702
Background Reference 1:
Tanaka N, Asao H, Ohtani K, Nakamura M, Sugamura K. A novel human tyrosine kinase gene inducible in T cells by interleukin 2. FEBS Lett 1993 Jun 7;324(1):1-5

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-784

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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