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Western blot analysis of extracts of various cell lines, using ITPA antibody (14-317) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using ITPA antibody (14-317) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

ITPA Antibody

CATALOG NUMBER: 14-317

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human ITPA (NP_258412.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
U-87MG
Positive Control 2:
HT-29
Positive Control 3:
Mouse small intestine
Positive Control 4:
Mouse heart
Positive Control 5:
Rat liver
Predicted Molecular Weight:
Observed: 21kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ITPA
Additional Names:
Inosine triphosphate pyrophosphatase {ECO:0000255, HAMAP-Rule:MF_03148}, ITPase {ECO:0000255, HAMAP-Rule:MF_03148}, Inosine triphosphatase {ECO:0000255, HAMAP-Rule:MF_03148}, 36119 {ECO:0000255, HAMAP-Rule:MF_03148}, Non-canonical purine NTP pyrophosphatase {ECO:0000255, HAMAP-Rule:MF_03148}, Non-standard purine NTP pyrophosphatase {ECO:0000255, HAMAP-Rule:MF_03148}, Nucleoside-triphosphate diphosphatase {ECO:0000255, HAMAP-Rule:MF_03148}, Nucleoside-triphosphate pyrophosphatase {ECO:0000255, HAMAP-Rule:MF_03148}, NTPase {ECO:0000255, HAMAP-Rule:MF_03148}, Putative oncogene protein hlc14-06-p, ITPA {ECO:0000255, HAMAP-Rule:MF_03148}, C20orf37
Ncbi Gene Id Number:
3704
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an inosine triphosphate pyrophosphohydrolase. The encoded protein hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. This protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency which causes an accumulation of ITP in red blood cells. Alternate splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-317

List Size:
100 uL

List Price:

$436.00

Datasheet
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