Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of lysates from A375, Hela, HepG2 cell line, mouse brain and rat liver tissue lysate(from left to right), using ITPA Antibody at 1:1000 at each lane.
Immunofluorescent analysis of Hela cells, using ITPA Antibody (N-term). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
ITPA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysates from A375, Hela, HepG2 cell line, mouse brain and rat liver tissue lysate(from left to right), using ITPA Antibody at 1:1000 at each lane.

ITPA Antibody

CATALOG NUMBER: 61-724

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat, Xenopus
Immunogen:
This ITPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human ITPA.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:25
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ITPA
Additional Names:
Inosine triphosphate pyrophosphatase {ECO:0000255, HAMAP-Rule:MF_03148}, ITPase {ECO:0000255, HAMAP-Rule:MF_03148}, Inosine triphosphatase {ECO:0000255, HAMAP-Rule:MF_03148}, 36119 {ECO:0000255, HAMAP-Rule:MF_03148}, Non-canonical purine NTP pyrophosphatase {ECO:0000255, HAMAP-Rule:MF_03148}, Non-standard purine NTP pyrophosphatase {ECO:0000255, HAMAP-Rule:MF_03148}, Nucleoside-triphosphate diphosphatase {ECO:0000255, HAMAP-Rule:MF_03148}, Nucleoside-triphosphate pyrophosphatase {ECO:0000255, HAMAP-Rule:MF_03148}, NTPase {ECO:0000255, HAMAP-Rule:MF_03148}, Putative oncogene protein hlc14-06-p, ITPA {ECO:0000255, HAMAP-Rule:MF_03148}, C20orf37
Protein Accession Number:
Q9BY32
Protein Gi Number:
30173120
Ncbi Gene Id Number:
3704
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ITPA hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. The encoded protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency. Two transcript variants encoding two different isoforms have been found for this gene.
Background Reference 1:
Fellay, J., et al. Nature 464(7287):405-408(2010)
Background Reference 2:
Herting, G., et al. Biochim. Biophys. Acta 1802(2):269-274(2010)
Background Reference 3:
Kudo, M., et al. Drug Metab. Pharmacokinet. 24(6):557-564(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-724

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369