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Western blot analysis of ITPKC Antibody in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ITPKC Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ITPKC Antibody in HepG2 cell line lysates (35ug/lane)

ITPKC Antibody

CATALOG NUMBER: 63-898

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ITPKC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the N-terminal region of human ITPKC.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
75 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ITPKC
Additional Names:
Inositol-trisphosphate 3-kinase C, Inositol 1,5-trisphosphate 3-kinase C, IP3 3-kinase C, IP3K C, InsP 3-kinase C, ITPKC, IP3KC
Protein Accession Number:
Q96DU7
Ncbi Gene Id Number:
80271
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ITPKC encodes a member of the inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)] 3-kinase family of enzymes that catalyze the phosphorylation of inositol 1,4,5-trisphosphate to 1,3,4,5-tetrakisphosphate. The encoded protein is localized to the nucleus and cytoplasm and has both nuclear import and nuclear export activity. Single nucleotide polymorphisms in this gene are associated with Kawasaki disease.
Background Reference 1:
Chi,H. Hum. Mol. Genet. 19 (6), 1147-1151 (2010)
Background Reference 2:
Onouchi,Y. Nat. Genet. 40 (1), 35-42 (2008)
Background Reference 3:
Nalaskowski,M.M. Biol. Chem. 387 (5), 583-593 (2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-898

List Size:
400 ul

List Price:

$495.00

Datasheet
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