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Western blot analysis of JAG1 in HeLa cell lysate with JAG1 antibody at 1 μg/ml.
Immunohistochemistry of JAG1 in human spleen tissue with JAG1 antibody at 5 μg/ml.
Immunofluorescence of JAG1 in human spleen tissue with JAG1 antibody at 20 μg/ml.
Western blot analysis of JAG1 in HeLa cell lysate with JAG1 antibody at 1 μg/ml.
Trial Size

JAG1 Antibody

CATALOG NUMBER: 8059

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
JAG1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human JAG1.
The immunogen is located within the last 50 amino acids of JAG1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
JAG1 antibody can be used for detection of JAG1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
JAG1 antibody is human, mouse and rat reactive. At least two isoforms of JAG1 are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 134 kDa
Observed: 130 kDa
Purification:
JAG1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
JAG1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
JAG1 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
JAG1
Additional Names:
Jagged 1, AGS, AHD, AWS, CD339, HJ1, JAGL1
Protein Accession Number:
NP_000205
Protein Gi Number:
4557679
Ncbi Gene Id Number:
182
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The LIN-12/Notch family of transmembrane receptors plays a central role in development by regulating cell fate decisions (1). Ligands for Notch include JAG1, JAG2 and Delta. JAG1 is one of the DSL (Delta, Serrate, Lag2) transmembrane proteins that can activate Notch. It is involved in mammalian cardiovascular development and in cell-fate decisions during hematopoiesis (1,2). JAG1 is widely expressed in adult and fetal tissues and the mutation of the JAG1 gene is associated with Familial Tetrology of Fallot (3). JAG1 expression is associated with prostate cancer metastasis and is up-regulated in cervical squamous cell carcinoma (4,5).
Background Reference 1:
Lai EC. Notch signaling: control of cell communication and cell fate. Development 2004; 131:965-73.
Background Reference 2:
Milner LA, Kopan R, Martin DI, et al. A human homologue of the Drosophila developmental gene, Notch, is expressed in CD34+ hematopoietic precursors. Blood1994; 83:2057-62.
Background Reference 3:
Guida V, Chiappe F, Ferese R, et al. Novel and recurrent JAG1 mutations in patients with tetralogy of Fallot. Clin. Genet. 2011; 80:591-4.
Background Reference 4:
Santagata S, Demichelis F, Riva A, et al. JAGGED1 expression is associated with prostate cancer metastasis and recurrence. Cancer Res. 2004; 64:6854-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8059

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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