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JARID2 Antibody

CATALOG NUMBER: 42-244

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-HRSAQDLRKQVSKVN
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 29kDa band in Mouse and Rat Spinal Cord and in Mouse Fetal Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 137kDa according to Mouse NP_068678.1. The 29kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
JARID2
Additional Names:
JARID2, jumonji, AT rich interactive domain 2, JMJ, OTTHUMP00000016058, jumonji homolog, jumonji, AT rich interactive domain 2 protein, jumonji-like protein
Protein Accession Number:
NP_004964.2
Protein Gi Number:
11863152
Ncbi Gene Id Number:
3720
Background Reference 1:
Bovill E, Westaby S, Reji S, Sayeed R, Crisp A, Shaw T. Induction by left ventricular overload and left ventricular failure of the human Jumonji gene (JARID2) encoding a protein that regulates transcription and reexpression of a protective fetal program. The Journal of thoracic and cardiovascular surgery 2008 Sep 136 (3): 709-16.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-244

List Size:
0.1 mg

List Price:

$391.00

Datasheet
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