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Western blot analysis of anti-JHDM1a/FBXL11 Antibody pre-incubated with(right lane) and without(left lane) blocking peptide in Jurkat cell line lysate.
Western blot analysis of anti-JHDM1a/FBXL11 Antibody pre-incubated with(right lane) and without(left lane) blocking peptide in Jurkat cell line lysate.

JHDM1a/FBXL11 Antibody

CATALOG NUMBER: 55-179

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This JHDM1a/FBXL11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 867-896 amino acids from the C-terminal region of human JHDM1a/FBXL11.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
133 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KDM2A
Additional Names:
Lysine-specific demethylase 2A, CXXC-type zinc finger protein 8, F-box and leucine-rich repeat protein 11, F-box protein FBL7, F-box protein Lilina, F-box/LRR-repeat protein 11, JmjC domain-containing histone demethylation protein 1A, [Histone-H3]-lysine-36 demethylase 1A, KDM2A, CXXC8, FBL7, FBXL11, JHDM1A, KIAA1004
Protein Accession Number:
Q9Y2K7
Protein Gi Number:
38257795
Ncbi Gene Id Number:
22992
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
JHDM1a/FBXL11 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box),which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. JHDM1a/FBXL11 belongs to the Fbls class and, in addition to an F-box, contains at least 6 highly degenerated leucine-rich repeats.
Background Reference 1:
Tsukada,Y.,Nature 439 (7078), 811-816 (2006)
Background Reference 2:
Andersen,J.S.,Nature 433 (7021), 77-83 (2005)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-179

List Size:
400 ul

List Price:

$495.00

Datasheet
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