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45-787 (0.1ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-787 (2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-787 (0.1ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

JIK Antibody

CATALOG NUMBER: 45-787

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: CHKKDHVFIRDEAGHGD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in human Liver lysate (calculated MW of 105kDa according to NP_057365). Recommended concentration: 0.3-1.0 ug/ml.Immunohistochemistry:In paraffin embedded Human Prostate shows staining of cytoplasm of secretory cells in the glands. Recommended concentration, 2-3ug/ml.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TAOK3
Additional Names:
TAOK3, JIK, TAO kinase 3, DPK, STE2-like kinase, DKFZp666H245, FLJ31808, MAP3K18, CTCL-associated antigen HD-CL-09, JNK/SAPK-inhibitory kinase, STE20-like kinase, cutaneous T-cell lymphoma-associated antigen HD-CL-09, dendritic cell-derived protein kinase, hKFC-A, jun kinase-inhibitory kinase, kinase from chicken homolog A, serine/threonine-protein kinase TAO3, thousand and one amino acid protein 3
Protein Accession Number:
NP_057365.3
Protein Gi Number:
148746218
Ncbi Gene Id Number:
51347
Background Reference 1:
Tassi E, Biesova Z, Di Fiore PP, Gutkind JS, Wong WT. Human JIK, a novel member of the STE20 kinase family that inhibits JNK and is negatively regulated by epidermal growth factor. J Biol Chem. 1999 Nov 19;274(47):33287-95.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-787

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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