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45-788 (4ug/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
45-788 (4ug/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

JIP3 Antibody

CATALOG NUMBER: 45-788

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Cow
Immunogen:
The immunogen for this antibody is: MEIQMDEGGGVV-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:No signal obtained yet but low background observed in Human Heart lysate at up to 2ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Mouse Brain shows axonal staining in various regions. Recommended concentration, 4-6ug/ml.
Specificity:
This antibody is expected to recognise both reported Human isoforms of this protein (as represented by NP_055948.2 and NP_001035529.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MAPK8IP3
Additional Names:
MAPK8IP3, mitogen-activated protein kinase 8 interacting protein 3, JIP3, SYD2, JSAP1, FLJ00027, KIAA1066, JNK-interacting protein 3, JNK/SAPK-associated protein-1, similar to sperm specific protein, homolog of Drosophila Sunday driver 2, JNK/stress-activated protein kinase-associated protein 1, AC012180.8, DKFZp762N1113, C-jun-amino-terminal kinase interacting protein 3, homolog of Drosophila Sunday driver 2
Protein Accession Number:
NP_055948.2,NP_001035529.1
Protein Gi Number:
14719398
Ncbi Gene Id Number:
23162
Background Reference 1:
Kelkar N, Gupta S, Dickens M, Davis RJ. Interaction of a mitogen-activated protein kinase signaling module with the neuronal protein JIP3. Mol Cell Biol. 2000 Feb;20(3):1030-43.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-788

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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