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Western blot analysis of SPAG9 using rabbit polyclonal SPAG9 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPAG9 gene (Lane 2).
Formalin-fixed and paraffin-embedded human testis tissue reacted with SPAG9 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of SPAG9 using rabbit polyclonal SPAG9 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPAG9 gene (Lane 2).

JLP Antibody

CATALOG NUMBER: 62-795

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This JLP (SPAG9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-120 amino acids from the N-terminal region of human JLP (SPAG9).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
146 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPAG9
Additional Names:
C-Jun-amino-terminal kinase-interacting protein 4, JIP-4, JNK-interacting protein 4, Cancer/testis antigen 89, CT89, Human lung cancer oncogene 6 protein, HLC-6, JNK-associated leucine-zipper protein, JLP, Mitogen-activated protein kinase 8-interacting protein 4, Proliferation-inducing protein 6, Protein highly expressed in testis, PHET, Sperm surface protein, Sperm-associated antigen 9, Sperm-specific protein, Sunday driver 1, SPAG9, HSS, KIAA0516, MAPK8IP4, SYD1
Protein Accession Number:
O60271
Ncbi Gene Id Number:
9043
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPAG9, which is abundantly expressed in testicular haploid germ cells, is recognized by sperm-agglutinating antibodies and implicated in infertility.
Background Reference 1:
Rana,R., Hum. Reprod. 21 (11), 2894-2900 (2006)
Background Reference 2:
Rana,R., Biochem. Biophys. Res. Commun. 340 (1), 158-164 (2006)
Background Reference 3:
Jagadish,N., KeioMed 54 (2), 66-71 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-795

List Size:
400 ul

List Price:

$495.00

Datasheet
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