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Western blot analysis of JMJD2A in rat liver tissue lysate with JMJD2A antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of JMJD2A in rat liver tissue with JMJD2A antibody at 5 μg/mL.
Western blot analysis of JMJD2A in rat liver tissue lysate with JMJD2A antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

JMJD2A Antibody

CATALOG NUMBER: 5373

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
JMJD2A antibody was raised against a 14 amino acid synthetic peptide from near the center of human JMJD2A.
The immunogen is located within amino acids 340 - 390 of JMJD2A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
JMJD2A antibody can be used for detection of JMJD2A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Purification:
JMJD2A Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
JMJD2A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
JMJD2A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KDM4A
Additional Names:
JMJD2A Antibody: JMJD2, JHDM3A, JMJD2A, TDRD14A, JMJD2, KIAA0677, Lysine-specific demethylase 4A, JmjC domain-containing histone demethylation protein 3A
Protein Accession Number:
CAH71021
Protein Gi Number:
55665236
Ncbi Gene Id Number:
9682
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
JMJD2A Antibody: Members of the Jumonji domain 2 (JMJD2) family contain a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. The first member of this group, JMJD2A, is widely expressed in human tissues and cell lines and functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. JMJD2A can also form a complex with the androgen receptor (AR), a transcription factor that is pivotal for the development of prostate cancer. Overexpression of JMJD2A stimulates AR function and this stimulation is dependent on JMJD2A catalytic activity, suggesting that JMJD2A might be a critical protein with roles in cell proliferation and oncogenesis.
Background Reference 1:
Gray SG, Iglesias AH, Lizcano F, et al. Functional characterization of JMJD2A, a histone deacetylase- and retinoblastoma-binding protein. J. Biol. Chem.2005; 31:28507-18.
Background Reference 2:
Shin S and Janknecht R. Activation of androgen receptor by histone demethylases JMJD2A and JMJD2D. Biochem. Biophys. Res. Comm.2007; 359:742-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5373

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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