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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

JMJD3 Antibody

CATALOG NUMBER: 29-186

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human JMJD3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
JMJD3 antibody can be used for detection of JMJD3 by ELISA at 1:1562500. JMJD3 antibody can be used for detection of JMJD3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
70 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store JMJD3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
KDM6B
Additional Names:
JMJD3, KIAA0346, JMJD3
Protein Accession Number:
AAH09994
Protein Gi Number:
14603031
Ncbi Gene Id Number:
23135
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
JMJD3 is a histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. It plays a central role in regulation of posterior development, by regulating HOX gene expression. It is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-186

List Size:
100 ul

List Price:

$473.00

Datasheet
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