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Western blot analysis of JMJD4 in human spleen tissue lysate with JMJD4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of JMJD4 in rat spleen tissue with JMJD4 antibody at 2.5 μg/mL.
Immunofluorescence of JMJD4 in rat spleen tissue with JMJD4 antibody at 5 μg/mL.
Western blot analysis of JMJD4 in human spleen tissue lysate with JMJD4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

JMJD4 Antibody

CATALOG NUMBER: 5379

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (71%)
Immunogen:
JMJD4 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human JMJD4.
The immunogen is located within amino acids 130 - 180 of JMJD4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
JMJD4 antibody can be used for detection of JMJD4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
This antibody will not recognize other members of the JMJD family.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
JMJD4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
JMJD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
JMJD4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
JMJD4
Additional Names:
JMJD4 Antibody: JmjC domain-containing protein 4, Jumonji domain-containing protein 4
Protein Accession Number:
NP_075383
Protein Gi Number:
239788847
Ncbi Gene Id Number:
65094
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
JMJD4 Antibody: The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. While several JMJD proteins have been identified as being involved in chromatin regulation, histone demethylation and development, the JMJD4 has not been identified. JMJD4 is expressed in multiple tissues and At least three isoforms of JMJD4 are known to exist.
Background Reference 1:
Takeuchi T, Watanabe Y, Takano-Shimizu T, et al. Roles of jumonji and jumonji family genes in chromatin regulation and development. Dev. Dyn.2006; 235:2449-59.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5379

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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