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Western blot analysis of JMJD7 in 3T3 cell lysate with JMJD7 antibody at 1 μg/mL.
Immunohistochemistry of JMJD7 in rat liver tissue with JMJD7 antibody at 2.5 μg/mL.
Immunofluorescence of JMJD7 in rat liver tissue with JMJD7 antibody at 20 μg/mL.
Western blot analysis of JMJD7 in 3T3 cell lysate with JMJD7 antibody at 1 μg/mL.
Trial Size

JMJD7 Antibody

CATALOG NUMBER: 5385

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
JMJD7 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human JMJD7.
The immunogen is located within amino acids 230 - 280 of JMJD7.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
JMJD7 antibody can be used for detection of JMJD7 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Purification:
JMJD7 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
JMJD7 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
JMJD7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
JMJD7
Additional Names:
JMJD7 Antibody: JmjC domain-containing protein 7, Jumonji domain-containing protein 7
Protein Accession Number:
P0C870
Protein Gi Number:
205783894
Ncbi Gene Id Number:
100137047
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
JMJD7 Antibody: The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. While several JMJD proteins have been identified as being involved in chromatin regulation, histone demethylation and development, the function of JMJD7 has not been identified. JMJD7 was initially thought to be a novel splice form of the phospholipase PLA2G4B which is located downstream of JMJD7 as most tissues express a read-through transcript of the two genes.
Background Reference 1:
Takeuchi T, Watanabe Y, Takano-Shimizu T, et al. Roles of jumonji and jumonji family genes in chromatin regulation and development. Dev. Dyn.2006; 235:2449-59.
Background Reference 2:
Clissold PM and Ponting CP. JmjC: cupin metalloenzyme-like domains in jumonji, hairless and phospholipase A2beta. Trends Biochem.Sci. 2001; 26:7-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5385

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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