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Western blot analysis of JPH1 in 293 cell lysate with JPH1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of JPH1 in mouse skeletal muscle tissue with JPH1 antibody at 2.5 μg/mL.
Western blot analysis of JPH1 in 293 cell lysate with JPH1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

JPH1 Antibody

CATALOG NUMBER: 4917

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (87%)
Immunogen:
JPH1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human JPH1.
The immunogen is located within amino acids 470 - 520 of JPH1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
JPH1 antibody can be used for detection of JPH1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Purification:
JPH1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
JPH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
JPH1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
JPH1
Additional Names:
JPH1 Antibody: JP1, JP-1, JP1, Junctophilin-1, Junctophilin type 1
Protein Accession Number:
AAI39833
Protein Gi Number:
145337941
Ncbi Gene Id Number:
56704
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
JPH1 Antibody: Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain and contribute to the subsurface cistern formation in neurons. JPH1 is essential for stabilizing the T-tubule and SR membranes to form junctions and provide an environment for the assembly of receptors such as the ryanodine receptor type 1 (RyR1).
Background Reference 1:
Takeshima H, Komazaki S, Nishi M, et al. Junctophilins: a novel family of junctional membrane complex proteins. Mol. Cell.2000; 6:11-22.
Background Reference 2:
Kakizawa S, Kishimoto Y, Hashimoto K, et al. Junctophilin-mediated channel crosstalk essential for cerebellar synaptic plasticity. EMBO J.2007; 26:1924-33.
Background Reference 3:
Nishi M, Sakagami H, Komazaki S, et al. Coexpression of junctophilin type 3 and type 4 in brain. Brain Res. Mol. Brain Res.2003; 118:102-10.
Background Reference 4:
Phimister AJ, Lango J, Lee EH, et al. Conformation-dependent stability of Junctophilin 1 (JP1) and Ryanodine Receptor type 1 (RyR1) channel complex is mediated by their hyper-reactive thiols. J. Biol. Chem.2007; 282:8867-77.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4917

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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