Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis in HepG2 cell line lysates (35ug/lane).
Fluorescent confocal image of U251 cell stained with JUN Antibody (Center T93).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with JUN primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). JUN immunoreactivity is localized to Nucleus significantly.
Western blot analysis in HepG2 cell line lysates (35ug/lane).

JUN Antibody

CATALOG NUMBER: 60-201

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Immunogen:
This JUN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-98 amino acids from the Central region of human JUN.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
JUN
Additional Names:
Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, JUN
Protein Accession Number:
P05412
Protein Gi Number:
135298
Ncbi Gene Id Number:
3725
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is the putative transforming gene of aviansarcoma virus 17. It encodes a protein which is highly similar tothe viral protein, and which interacts directly with specifictarget DNA sequences to regulate gene expression. This gene isintronless and is mapped to 1p32-p31, a chromosomal region involvedin both translocations and deletions in human malignancies.
Background Reference 1:
Gonsalves, C., et al. J. Immunol. 185(10):6253-6264(2010)
Background Reference 2:
Bozec, A., et al. J. Cell Biol. 190(6):1093-1106(2010)
Background Reference 3:
Machida, K., et al. Hepatology 52(2):480-492(2010)
Background Reference 4:
Madi, A., et al. BMC Microbiol. 10, 215 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-201

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369