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Western blot analysis in PC-3U87-MG cell line lysates (35ug/lane).This demonstdetected the JUN protein (arrow).
Western blot analysis in PC-3U87-MG cell line lysates (35ug/lane).This demonstdetected the JUN protein (arrow).

JUN Antibody

CATALOG NUMBER: 60-744

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This JUN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from human JUN.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
JUN
Additional Names:
Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, JUN
Protein Accession Number:
P05412
Protein Gi Number:
135298
Ncbi Gene Id Number:
3725
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
JUN interacts directly with specific target DNA sequences to regulate gene expression. Jun recognizes the AP-1 consensus sequence TGACTCA, a response element that confers sensitivity to one of the tumor-promoting Phorbol esters, 12-O-tetradecanoyl-phorbol-13-acetate (see also: TRE, TPA response element). Jun itself forms homodimers or heterodimers with junD and junB and also interacts with the oncogene product fos, forming jun-fos heterodimers.
Background Reference 1:
Fujita,S., J. Mol. Biol. 378 (3), 492-504 (2008)
Background Reference 2:
Gan,X.Q.,Cell Biol. 180 (6), 1087-1100 (2008)
Background Reference 3:
Yogev,O., Cancer Res. 68 (5), 1398-1406 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-744

List Size:
400 ul

List Price:

$495.00

Datasheet
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