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Western blot analysis of lysates from Mouse brain, using JUNB Rabbit mAb at 1:2000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit. | Exposure time: 45s.
Western blot analysis of lysates from Mouse brain, using JUNB Rabbit mAb at 1:2000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit. | Exposure time: 45s.

JUNB Rabbit Recombinant Antibody (82-521)

CATALOG NUMBER: 82-521

Clonality:
Monoclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
WB,1:1000 - 1:5000, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Mouse brain
Predicted Molecular Weight:
36kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
JUNB
Additional Names:
AP-1; JUNB
Protein Accession Number:
P17275
Ncbi Gene Id Number:
3726
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables sequence-specific double-stranded DNA binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of transcription factor AP-1 complex. Biomarker of Hodgkin's lymphoma and anaplastic large cell lymphoma.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-521

List Size:
100 uL

List Price:

$477.00

Datasheet
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