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43-498 (1ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-498 (1ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

JUND Antibody

CATALOG NUMBER: 43-498

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog
Immunogen:
The immunogen for this antibody is: CQLLPQHQVPAY
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 35.2kDa according to NP_005345.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Short and Pfarr,J Biol Chem. 2002 Sep 6;277(36):32697-705. PMID: 12105216). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected NOT to cross-react with Jun oncogen (NP_002219.1, GeneID 3725)
Predicted Molecular Weight:
Approx 40kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
JUND
Additional Names:
JUND, jun D proto-oncogene , JunD-FL isoform, transcription factor jun-D
Protein Accession Number:
NP_005345.3
Protein Gi Number:
102469037
Ncbi Gene Id Number:
3727
Background Reference 1:
Hartman TR, Qian S, Bolinger C, Fernandez S, Schoenberg DR, Boris-Lawrie K. RNA helicase A is necessary for translation of selected messenger RNAs.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-498

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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