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Western blot analysis of anti-DH6 Antibody in 293 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CDH6 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of NCI-H292 cells using CDH6 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of anti-DH6 Antibody in 293 cell line lysates (35ug/lane)

K Cadherin Antibody

CATALOG NUMBER: 57-524

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This K Cadherin (CDH6) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 665-695 amino acids from the C-terminal region of human K Cadherin (CDH6).
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
88 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDH6
Additional Names:
Cadherin-6, Kidney cadherin, K-cadherin, CDH6
Protein Accession Number:
P55285
Protein Gi Number:
1705545
Ncbi Gene Id Number:
1004
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CDH6 is a type II classical cadherin from the cadherin superfamily. It is a calcium dependent cell-cell adhesion membrane glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Cadherins mediate cell-cell binding in a homophilic manner, contributing to the sorting of heterogeneous cell types and the maintenance of orderly structures such as epithelium. Strong transcriptional expression of the CDH6 gene has been observed in hepatocellular and renal carcinoma cell lines, suggesting a possible role in metastasis and invasion.
Background Reference 1:
Liu,T., J. Proteome Res.(6), 2070-2080 (2005)
Background Reference 2:
Shimoyama,Y., Biochem. J. 349 (PT 1), 159-167 (2000)
Background Reference 3:
Shimoyama,Y., J. Biol. Chem. 274 (17), 11987-11994 (1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-524

List Size:
400 ul

List Price:

$495.00

Datasheet
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