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Western blot analysis of K2C78 Antibody in Hela cell line lysates (35ug/lane)
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of K2C78 Antibody in Hela cell line lysates (35ug/lane)

K2C78 Antibody

CATALOG NUMBER: 64-150

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This K2C78 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-203 amino acids from the Central region of human K2C78.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KRT78
Additional Names:
Keratin, type II cytoskeletal 78, Cytokeratin-78, CK-78, Keratin-5b, Keratin-78, K78, Type-II keratin Kb40, KRT78, K5B, KB40
Protein Accession Number:
Q8N1N4
Protein Gi Number:
166218809
Ncbi Gene Id Number:
196374
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the type II keratin gene family and encodes a protein with an intermediate filament domain. Keratins are the major structural proteins in epithelial cells, forming a cytoplasmic network of 10 to 12 nm wide intermediate filaments and creating a scaffold that gives cells the ability to withstand mechanical and non-mechanical stresses. The genes of the type II keratin family are located as a gene cluster at 12p13.13. Four pseudogenes of this gene family have been identified.
Background Reference 1:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Background Reference 2:
Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006)
Background Reference 3:
Rogers, M.A., et al. J. Invest. Dermatol. 124(3):536-544(2005)
Background Reference 4:
Hesse, M., et al. Eur. J. Cell Biol. 83(1):19-26(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-150

List Size:
400 ul

List Price:

$495.00

Datasheet
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