K562 Cytoplasmic Lysate

CATALOG NUMBER: XBL-10447

Tested Applications:
WB
Specifications
Tissue / Cell Type:
K-562
Tissue Cell Type Expanded:
K562 (Human Chronic Myelogenous Leukemia; Bone Marrow) Cell Line
Species:
Human
diagnosis:
Tumor
Tested Applications:
WB
Application Note:
K562 (Chronic Myelogenous Leukemia; Bone Marrow) Cell Cytoplasmic protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
Physical State:
Liquid
Buffer:
HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
Concentration:
2 mg/mL or better
Storage Conditions:
Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Background:
K562 (Human Chronic Myelogenous Leukemia; Bone Marrow) Cell cytoplasmic Protein Lysate cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated K562 (Human Chronic Myelogenous Leukemia; Bone Marrow) cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated K562 (Human Chronic Myelogenous Leukemia; Bone Marrow) tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XBL-10447

List Size:
0.1 mg

List Price:

$369.00

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