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45-789 (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-789 (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Kalirin Antibody

CATALOG NUMBER: 45-789

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse,
Immunogen:
The immunogen for this antibody is: C-RWHLGPGDPFSTYV
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 200kDa band observed in Rat Brain lysates (calculated MW of 192kDa according to NP_003938.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Specificity:
This antibody is expected to recognize isoform 2 (NP_003938.1).
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
KALRN
Additional Names:
KALRN, kalirin, RhoGEF kinase, FLJ16443, HAPIP, TRAD, duo, huntingtin-associated protein interacting protein (duo), serine/threonine kinase with Dbl- and pleckstrin homology domains, hCG_2022551, DUET, DUO, FLJ12332, FLJ18196, FLJ18623,
Protein Accession Number:
NP_003938.1
Protein Gi Number:
4504335
Ncbi Gene Id Number:
8997
Background Reference 1:
Colomer V, Engelender S, Sharp AH, Duan K, Cooper JK, Lanahan A, Lyford G, Worley P, Ross CA. Huntingtin-associated protein 1 (HAP1) binds to a Trio-like polypeptide, with a rac1 guanine nucleotide exchange factor domain. Hum Mol Genet. 1997 Sep;6(9):1519-25.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-789

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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