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Kanamycin kinase type II Recombinant Protein

CATALOG NUMBER: 92-658

Specifications
source-species:
E. coli
Species:
K. pneumoniae
source-species:
E. coli
Recombinant Protein Sequence:
Met1-Phe264
Fusion Tag:
Tag Free
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Predicted Molecular Weight:
29 kD
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.
Storage Conditions:
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at -20°C for 3 months.
Ncbi Official Symbol:
neo
Additional Names:
Aminoglycoside 3'-phosphotransferase, APH(3')-II, APH(3')II, Kanamycin kinase type II, Neomycin-kanamycin phosphotransferase type II, neo
Protein Accession Number:
P00552
Background:
Aminoglycoside 3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3') is primarily found in certain species of gram-positive bacteria.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

92-658

List Size:
0.05 mg

List Price:

$515.00

Datasheet
Shipping Info

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