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Western blot analysis of KANK1 in 3T3 cell lysate with KANK1 antibody at 1 μg/mL.
Immunohistochemistry of KANK1 in human kidney tissue with KANK1 antibody at 2.5 μg/ml.
Immunofluorescence of KANK1 in human kidney tissue with KANK1 antibody at 20 μg/ml.
Western blot analysis of KANK1 in 3T3 cell lysate with KANK1 antibody at 1 μg/mL.
Trial Size

KANK1 Antibody

CATALOG NUMBER: 7307

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
KANK1 antibody was raised against a 19 amino acid peptide near the amino terminus of human KANK1 .
The immunogen is located within the first 50 amino acids of KANK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
KANK1 Antibody can be used for detection of KANK1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Two alternatively spliced transcript variants encoding different isoforms have been identified. The lower molecular weight band seen in the immunoblot is thought to be non-specific.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
Predicted: 149 kDa
Observed: 150 kDa
Purification:
KANK1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KANK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
KANK1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KANK1
Additional Names:
KANK1 Antibody: KANK, CPSQ2, ANKRD15, KANK, KIAA0172, KN motif and ankyrin repeat domain-containing protein 1, Ankyrin repeat domain-containing protein 15
Protein Accession Number:
NP_055973
Protein Gi Number:
64464726
Ncbi Gene Id Number:
23189
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KANK1 Antibody: Ankyrins are membrane adaptor molecules that play important roles in the control of cytoskeleton formation by regulating actin polymerization. KANK1 (KN motif and ankyrin repeat domain-containing protein 1), also known as ANKRD15, is a 1,352 amino acid protein that contains at least 12 exons and 5 ANK repeats. It binds to beta-catenin and regulates its subcellular distribution. KANK1 is ubiquitously expressed and localizes to cytoplasm. It may function as a tumor suppressor for renal cell carcinoma. Mutations in this gene cause cerebral palsy spastic quadriplegic type 2, a central nervous system development disorder.
Background Reference 1:
Zhu Y, Kakinuma N, Wang Y, et al. Kank proteins: a new family of ankyrin-repeat domain containing proteins. Biochim. Biophys. Acta 2008; 1780:128-33.
Background Reference 2:
Roy BC, Kakinuma N, Kiyama R. Kank attenuates actin remodeling by preventing interaction between IRSp53 and Rac1. J. Cell Biol. 2009; 184:253-67.
Background Reference 3:
Sarkar S, Roy BC, Hatano N, et al. A novel ankyrin repeat-containing gene (Kank) located at 9p24 is a growth suppressor of renal cell carcinoma. J. Biol. Chem. 2002; 277:36585-91.
Background Reference 4:
Lerer I, Sagi M, Meiner V, et al. Deletion of the ANKRD15 gene at 9p24.3 causes parent-of-origin-dependent inheritance of familial cerebral palsy. Hum. Mol. Genet. 2005; 14: 3911-20

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7307

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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