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Western blot analysis of KappaB ras in RAW 264.7 cell lysate with KappaB ras antibody at 2 μg/mL.
Immunohistochemistry of KappaB ras in human lymph node tissue with KappaB ras antibody at 1 μg/mL.
Western blot analysis of KappaB ras in RAW 264.7 cell lysate with KappaB ras antibody at 2 μg/mL.
Green Product
Trial Size

KappaB ras Antibody

CATALOG NUMBER: 2491

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (94%)
Immunogen:
KappaB ras antibody was raised against a 18 amino acid synthetic peptide from near the center of human KappaB ras 1.
The immunogen is located within amino acids 80 - 130 of KappaB ras.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
KappaB ras antibody can be used for detection of KappaB ras by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1283 - RAW 264.7 Cell Lysate
Positive Control 2:
Cat. No. 1369 - Human Lymph node Tissue Lysate
Positive Control 3:
Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
Purification:
KappaB ras Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
KappaB ras Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
KappaB ras antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NKIRAS1
Additional Names:
KappaB ras Antibody: KBRAS1, kappaB-Ras1, KBRAS1, NF-kappa-B inhibitor-interacting Ras-like protein 1, I-kappa-B-interacting Ras-like protein 1, Kappa B-Ras protein 1
Protein Accession Number:
AAF34998
Protein Gi Number:
7008402
Ncbi Gene Id Number:
28512
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KappaB ras Antibody: KappaB ras-1 (κB-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IκB (IκB), an inhibitor of the transcription factor NF-κB. IκB exists in two homologous forms, IκB-alpha and IκB-beta, although IκB-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive IκB-alpha-NF-κB complexes can shuttle in and out of the nucleus, IκB-beta-NF-κB complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the IκB-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of IκB-beta and regulating the rate of degradation of IκB-beta. This antibody detects both kappaB-ras1 and kappaB-ras2.
Background Reference 1:
Fenwick C, Na SY, Voll RE, et al. A subclass of Ras proteins that regulate the degradation of IkappaB. Science 2000; 287:869-73.
Background Reference 2:
Chen Y, Wu J and Ghosh G. KappaB-Ras binds to the unique insert within the ankyrin repeat domain if IkappaBbeta and regulates cytoplasmic retention of IkappaBbeta x NF-kappaB complexes. J. Biol. Chem.2003; 278:23101-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2491

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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