Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of KappaB ras2 in RAW264.7 cell lysate with KappaB ras2 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Western blot analysis of KappaB ras2 in RAW264.7 cell lysate with KappaB ras2 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Trial Size

KappaB ras2 Antibody

CATALOG NUMBER: 2495

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (93%)
Immunogen:
KappaB ras2 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human KappaB ras2.
The immunogen is located within the last 50 amino acids of KappaB ras2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
KappaB ras2 antibody can be used for detection of KappaB ras1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1283 - RAW 264.7 Cell Lysate
Purification:
KappaB ras2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KappaB ras2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
KappaB ras2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NKIRAS2
Additional Names:
KappaB ras2 Antibody: KBRAS2, kappaB-Ras2, KBRAS2, NF-kappa-B inhibitor-interacting Ras-like protein 2, I-kappa-B-interacting Ras-like protein 2, Kappa B-Ras protein 2
Protein Accession Number:
NP_060065
Protein Gi Number:
19072794
Ncbi Gene Id Number:
28511
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KappaB ras2 Antibody: KappaB ras-1 (κB-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IκB (IκB), an inhibitor of the transcription factor NF-κB. IκB exists in two homologous forms, IκB-alpha and IκB-beta, although IκB-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive IκB-alpha-NF-κB complexes can shuttle in and out of the nucleus, IκB-beta-NF-κB complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the IκB-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of IκB-beta and regulating the rate of degradation of IκB-beta. This antibody is specific for kappaB-ras2 and has no cross-reactivity to kappaB-ras1.
Background Reference 1:
Fenwick C, Na SY, Voll RE, et al. A subclass of Ras proteins that regulate the degradation of IkappaB. Science 2000; 287:869-73.
Background Reference 2:
Chen Y, Wu J and Ghosh G. KappaB-Ras binds to the unique insert within the ankyrin repeat domain if IkappaBbeta and regulates cytoplasmic retention of IkappaBbeta x NF-kappaB complexes. J. Biol. Chem.2003; 278:23101-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2495

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369