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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western blot analysis of KARS using rabbit polyclonal KARS Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KARS gene.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

KARS Antibody

CATALOG NUMBER: 55-623

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This KARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human KARS.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KARS
Additional Names:
Lysine--tRNA ligase, Lysyl-tRNA synthetase, LysRS, KARS, KIAA0070
Protein Accession Number:
Q15046
Protein Gi Number:
20178333
Ncbi Gene Id Number:
3735
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Aminoacyl-tRNA synthetases are a class of enzymes thatcharge tRNAs with their cognate amino acids. Lysyl-tRNA synthetaseis a homodimer localized to the cytoplasm which belongs to theclass II family of tRNA synthetases. It has been shown to be atarget of autoantibodies in the human autoimmune diseases,polymyositis or dermatomyositis. Alternatively spliced transcriptvariants encoding different isoforms have been found for this gene.
Background Reference 1:
McLaughlin, H.M., et al. Am. J. Hum. Genet. 87(4):560-566(2010)
Background Reference 2:
Kepp, O., et al. Cell Cycle 9(15):3072-3077(2010)
Background Reference 3:
Segat, L., et al. Vaccine 28(10):2201-2206(2010)
Background Reference 4:
Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-623

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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