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Western blot analysis of KAT1 (HAT1) Antibody in HL60 cell line lysates (35ug/lane)
Western blot analysis of KAT1 (HAT1) Antibody in HL60 cell line lysates (35ug/lane)

KAT1 Antibody

CATALOG NUMBER: 55-428

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This KAT1 (HAT1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-58 amino acids from the N-terminal region of human KAT1 (HAT1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HAT1
Additional Names:
Histone acetyltransferase type B catalytic subunit, Histone acetyltransferase 1, HAT1, KAT1
Protein Accession Number:
O14929
Ncbi Gene Id Number:
8520
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histone acetylation, particularly of histone H4, has been proposed to play an important role in replication-dependent nucleosome assembly. The HAT1 protein contains D, A, and B motifs, which are present in many N-acetyltransferases, including those that acetylate substrates other than histones. The HAT1 holoenzyme consists of 2 subunits: the catalytic 46-kD HAT1 and the accessory p46. The p46 subunit stimulated the activity of HAT1 and bound to core histones. The HAT1 holoenzyme acetylated newly synthesized but not nucleosomal histone H4 at lys5 and lys12, and, to a lesser extent, histone H2A at lys5. HAT1 and p46 polypeptides are located in the nucleus of S-phase cells. HAT1 may play a role in telomeric silencing.
Background Reference 1:
Gronroos, E., et al., Mol. Cell 10(3):483-493 (2002).
Background Reference 2:
Makowski, A.M., et al., J. Biol. Chem. 276(47):43499-43502 (2001).
Background Reference 3:
Cheung, P., et al., Mol. Cell 5(6):905-915 (2000).
Background Reference 4:
Verreault, A., et al., Curr. Biol. 8(2):96-108 (1998).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-428

List Size:
400 ul

List Price:

$495.00

Datasheet
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