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Western blot analysis of extracts of various cell lines, using KCNE1 antibody (14-135) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using KCNE1 antibody (14-135) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

KCNE1 Antibody

CATALOG NUMBER: 14-135

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KCNE1 (NP_000210.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
H460
Positive Control 2:
293T
Positive Control 3:
U937
Positive Control 4:
Mouse brain
Predicted Molecular Weight:
Observed: 16kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KCNE1
Additional Names:
ISK, JLNS, JLNS2, LQT2/5, LQT5, MinK, potassium voltage-gated channel subfamily E member 1, IKs producing slow voltage-gated potassium channel subunit beta Mink, cardiac delayed rectifier potassium channel protein, delayed rectifier potassium channel subunit IsK, minimal potassium channel, potassium channel, voltage gated subfamily E regulatory beta subunit 1, potassium voltage-gated channel, Isk-related family, member 1, potassium voltage-gated channel, Isk-related subfamily, member 1, voltage gated potassiun channel accessory subunit
Ncbi Gene Id Number:
3753
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene belongs to the potassium channel KCNE family. Potassium ion channels are essential to many cellular functions and show a high degree of diversity, varying in their electrophysiologic and pharmacologic properties. This gene encodes a transmembrane protein known to associate with the product of the KVLQT1 gene to form the delayed rectifier potassium channel. Mutation in this gene are associated with both Jervell and Lange-Nielsen and Romano-Ward forms of long-QT syndrome. Alternatively spliced transcript variants encoding the same protein have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-135

List Size:
100 uL

List Price:

$436.00

Datasheet
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