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42-072 (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-072 (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

KCNJ1 Antibody

CATALOG NUMBER: 42-072

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DQININFVVDAGNEN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 45kDa band observed in Human Kidney lysates (calculated MW of 44.8kDa according to NP_000211.1). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize reported isoforms NP_722449.2 and NP_000211. The following reported variants represent identical protein: NP_722451.1, NP_722449.2, NP_722450.1, NP_722448.1.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
KCNJ1
Additional Names:
KCNJ1, potassium inwardly-rectifying channel, subfamily J, member 1, KIR1.1, ROMK, ROMK1, ATP-regulated potassium channel ROM-K, ATP-sensitive inward rectifier potassium channel 1, OTTHUMP00000045938, inwardly rectifying K+ channel, potassium inwardly-rectifying channel J1
Protein Accession Number:
NP_000211.1,NP_722448.1
Protein Gi Number:
4504837
Ncbi Gene Id Number:
3758
Background Reference 1:
Ji W, Foo JN, O1Roak BJ, Zhao H, Larson MG, Simon DB, Newton-Cheh C, State MW, Levy D, Lifton RP. Rare independent mutations in renal salt handling genes contribute to blood pressure variation. Nat. Genet. 2008 May 40 (5): 592-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-072

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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