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Western blot analysis of extracts of various cell lines, using KCNJ8 antibody (13-725) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Western blot analysis of extracts of various cell lines, using KCNJ8 antibody (13-725) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

KCNJ8 Antibody

CATALOG NUMBER: 13-725

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 325-424 of human KCNJ8 (NP_004973.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:1000 - 1:2000
Positive Control 1:
U-251MG
Positive Control 2:
K-562
Positive Control 3:
SW480
Predicted Molecular Weight:
Observed: 48kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KCNJ8
Additional Names:
KIR6.1, uKATP-1, ATP-sensitive inward rectifier potassium channel 8, inward rectifier K(+) channel Kir6.1, inwardly rectifying potassium channel KIR6.1, potassium channel, inwardly rectifying subfamily J member 8, potassium inwardly-rectifying channel, subfamily J, member 8
Ncbi Gene Id Number:
3764
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. Defects in this gene may be a cause of J-wave syndromes and sudden infant death syndrome (SIDS).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-725

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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