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Western blot analysis of extracts of various cell lines, using KCNK9 antibody (23-688) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human liver damage using KCNK9 antibody (23-688) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using KCNK9 antibody (23-688) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using KCNK9 antibody (23-688) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 15s.

KCNK9 Antibody

CATALOG NUMBER: 23-688

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KCNK9 (NP_001269463.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
293T
Positive Control 2:
Mouse kidney
Positive Control 3:
Rat kidney
Predicted Molecular Weight:
Observed: 42kDa/53kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KCNK9
Additional Names:
KCNK9, K2p9.1, KT3.2, MGC138268, MGC138270, TASK-3, TASK3
Ncbi Gene Id Number:
51305
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that contains multiple transmembrane regions and two pore-forming P domains and functions as a pH-dependent potassium channel. Amplification and overexpression of this gene have been observed in several types of human carcinomas. This gene is imprinted in the brain, with preferential expression from the maternal allele. A mutation in this gene was associated with Birk-Barel mental retardation dysmorphism syndrome. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-688

List Size:
100 uL

List Price:

$436.00

Datasheet
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