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Western blot analysis of extracts of various cell lines, using KCNMB2 antibody (13-551) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human appendix using KCNMB2 antibody (13-551) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using KCNMB2 antibody (13-551) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

KCNMB2 Antibody

CATALOG NUMBER: 13-551

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 68-194 of human KCNMB2 (NP_005823.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Mouse kidney
Positive Control 2:
Mouse brain
Positive Control 3:
Mouse lung
Positive Control 4:
Rat brain
Positive Control 5:
Rat kidney
Predicted Molecular Weight:
Observed: 30kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KCNMB2
Additional Names:
calcium-activated potassium channel subunit beta-2, BK channel beta subunit 2, BK channel subunit beta-2, MaxiK channel beta 2 subunit, MaxiK channel beta-subunit 2, big potassium channel beta subunit 2, charybdotoxin receptor subunit beta-2, hCG1646471, hbeta2, k(VCA)beta-2, large conductance calcium-activated potassium channel beta 2 subunit, large-conductance Ca2+-activated K+ channel beta2 subunit, potassium channel subfamily M regulatory beta subunit 2, potassium large conductance calcium-activated channel, subfamily M, beta member 2, slo-beta-2
Ncbi Gene Id Number:
10242
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which decreases the activation time of MaxiK alpha subunit currents. Alternative splicing results in multiple transcript variants of this gene. Additional variants are discussed in the literature, but their full length nature has not been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-551

List Size:
100 uL

List Price:

$436.00

Datasheet
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