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Western blot analysis of KCTD15 in HeLa cell lysate with KCTD15 antibody at 1μg/ml in (A) the absence and (B) the presence of blocking buffer.
Immunohistochemistry of KCTD15 in human spleen tissue with KCTD15 antibody at 2.5 μg/mL.
Immunofluorescence of KCTD15 in human spleen tissue with KCTD15 antibody at 20 μg/mL.
Western blot analysis of KCTD15 in HeLa cell lysate with KCTD15 antibody at 1μg/ml in (A) the absence and (B) the presence of blocking buffer.
Trial Size

KCTD15 Antibody

CATALOG NUMBER: 5085

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
KCTD15 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human KCTD15.
The immunogen is located within the last 50 amino acids of KCTD15.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
KCTD15 antibody can be used for detection of KCTD15 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
KCTD15 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KCTD15 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
KCTD15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KCTD15
Additional Names:
KCTD15 Antibody: Potassium channel tetramerization domain-containing protein 15
Protein Accession Number:
Q96SI1
Protein Gi Number:
74732704
Ncbi Gene Id Number:
79047
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KCTD15 Antibody: Childhood and adult obesity in the United States and to a lesser extent the rest of the world has increased dramatically over the past decade. Both environmental and genetic factors are involved in the onset and progression of weight gain. Recently, the potassium channel KCTD15 was identified as a genetic loci associated with higher than normal body mass index (BMI) in humans along with genes such as GNPDA2, MTCH2, FTO, and TMEM18. Further studies on single nucleotide polymorphisms (SNPs) in non-diabetic and diabetic patients showed that FTO was most strongly associated with obesity while MTCH2 and GNPDA2 were still significantly associated with higher than normal BMI levels. At least two isoforms of KCTD15 are known to exist.
Background Reference 1:
Hill JO. Genetic and environmental contributions to obesity. Am. J. Clin. Nutr.1998; 68:991-2.
Background Reference 2:
Willer CJ, Speliotes EK, Loos RJ, et al. Six new loci associated with body mass index highlight a neuronal influence on body weight regulation. Nat. Genetics2009; 41:25-34.
Background Reference 3:
Renstrom F, Payne F, Nordstrom A, et al. Replication and extension of genome-wide association study results for obesity in 4923 adults from northern Sweden. Human. Mol. Gen.2009; 18:1489-96.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5085

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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