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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
KCTD3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.

KCTD3 Antibody

CATALOG NUMBER: 56-985

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This KCTD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 725-754 amino acids from the C-terminal region of human KCTD3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
89 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KCTD3
Additional Names:
BTB/POZ domain-containing protein KCTD3, Renal carcinoma antigen NY-REN-45, KCTD3
Protein Accession Number:
Q9Y597
Protein Gi Number:
112823993
Ncbi Gene Id Number:
51133
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KCTD3 belongs to the KCTD3 family. It contains one BTB (POZ) domain and five WD repeats. The exact function of KCTD3 is not known. There are three named isoforms.
Background Reference 1:
Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)
Background Reference 2:
Scanlan, M.J., et al. Int. J. Cancer 83(4):456-464(1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-985

List Size:
400 ul

List Price:

$495.00

Datasheet
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