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Western blot analysis of lysates from wild type (WT) and Bcl-2 knockdown (KD) 293T cells using [KD Validated] Bcl-2 Rabbit mAb (A21873) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020) | Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using [KD Validated] Bcl-2 Rabbit mAb (A21873) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human follicular lymphoma tissue using [KD Validated] Bcl-2 Rabbit mAb (A21873) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] Bcl-2 Rabbit mAb (A21873) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg [KD Validated] Bcl-2 Rabbit mAb (A21873). Western blot was performed from the immunoprecipitate using [KD Validated] Bcl-2 Rabbit mAb (A21873) at a dilution of 1:1000.
Western blot analysis of lysates from wild type (WT) and Bcl-2 knockdown (KD) 293T cells using [KD Validated] Bcl-2 Rabbit mAb (A21873) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020) | Exposure time: 30s.

[KD Validated] Bcl-2 Rabbit Recombinant Antibody

CATALOG NUMBER: 81-994

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC-P, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, IP, WB
Application Note:
WB,1:1000 - 1:4000, IHC-P,1:200 - 1:800, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
293T,
Predicted Molecular Weight:
26kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
BCL2
Additional Names:
Bcl-2; PPP1R50
Protein Accession Number:
P10415
Ncbi Gene Id Number:
596
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-994

List Size:
100 uL

List Price:

$540.00

Datasheet
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