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Western blot analysis of various lysates, using [KD Validated] beta 2 Microglobulin Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.
Western blot analysis of lysates from wild type(WT) and β2-microglobulin knockdown (KD) HeLa cells, using [KD Validated] beta 2 Microglobulin Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] beta 2 Microglobulin Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KD Validated] beta 2 Microglobulin Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] beta 2 Microglobulin Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] beta 2 Microglobulin Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using [KD Validated] beta 2 Microglobulin Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Flow cytometry:1X10^6 Daudi cells(negative control,left) and Hela cells (right) were surface-stained with [KD Validated] beta Microglobulin Rabbit mAb(2 μg/mL,orange line) or PSFlo 647 Rabbit IgG isotype control.Non-fluorescently stained cells was used as blank control (red line).
Flow cytometry:1X10^6 HeLa cells were surface-stained with PSFlo 647 Rabbit IgG isotype control or β2 Microglobulin Rabbit mAb(2 μg/mL,right).
Western blot analysis of various lysates, using [KD Validated] beta 2 Microglobulin Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.

[KD Validated] beta 2 Microglobulin Rabbit Recombinant Antibody (82-601)

CATALOG NUMBER: 82-601

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IF/ICC, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IF/ICC, IHC-P, WB
Application Note:
WB,1:1000 - 1:4000, IHC-P,1:1000 - 1:4000, IF/ICC,1:50 - 1:200, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
13kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
B2M
Additional Names:
B2M; IMD43; beta-2-microglobulin; [KD Validated] beta 2 Microglobulin
Protein Accession Number:
P61769
Ncbi Gene Id Number:
567
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-601

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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