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Western blot analysis of various lysates, using [KD Validated] CD98/SLC3A2 Rabbit mAb (A24735) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 45s.
Western blot analysis of lysates from wild type (WT) and CD98/SLC3A2 knockdown (KD) HeLa cells using [KD Validated] CD98/SLC3A2 Rabbit mAb (A24735) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 15s.
Confocal imaging of HeLa cells using [KD Validated] CD98/SLC3A2 Rabbit mAb (A24735,dilution 1:200) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Confocal imaging of Hep G2 cells using [KD Validated] CD98/SLC3A2 Rabbit mAb (A24735,dilution 1:200) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Flow cytometry: 1X10^6 SH-SY5Y cells (Low Expression,left) and HeLa cells (right) were surface-stained with CD98/SLC3A2 Rabbit mAb (A24735,2 μg/mL,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 HeLa cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or CD98/SLC3A2 Rabbit mAb (A24735,2 μg/mL,right).
Western blot analysis of various lysates, using [KD Validated] CD98/SLC3A2 Rabbit mAb (A24735) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 45s.

[KD Validated] CD98/SLC3A2 Rabbit Recombinant Antibody

CATALOG NUMBER: 82-989

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IF/ICC, WB
Application Note:
WB,1:1000 - 1:4000, IF/ICC,1:200 - 1:2000, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
57kDa/61kDa/67kDa/68kDa/71kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SLC3A2
Additional Names:
4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC; CD98/SLC3A2
Protein Accession Number:
P08195
Ncbi Gene Id Number:
6520
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-989

List Size:
100 uL

List Price:

$540.00

Datasheet
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